Isolation, molecular characterization and functional analysis of OeMT2, an olive metallothionein with a bioremediation potential Isolation and characterization of chemo-resistant stem Figure 1 (A) Growth inhibitory effect of 5-FU on Mlh 1 /1638N COL-Cl 1 cells. The number of viable cells number was determined 7 days after seeding of 1.0×10 5 cells. The data are eed as the mean ± SD, n=3 per treatment group, and were analyzed by ANOVA and Dunnetts multiple comparison test (=0.05). 0.1 µM 5-FU P=0.047, 0.20 µM 5-FU P=0.038, 0.5 µM 5-FU P=0.010, relative to the
Isolation, characterization, and function analysis of a flavonol synthase gene from Ginkgo biloba Isolation, characterization, and functional groups Isolation, characterization, and functional groups analysis of Pseudoxanthomonas indica RSA-23 from rhizosphere soil Sreenivasa Nayaka*, Bidhayak Chakraborty, Pallavi Sathyanarayan Swamy, Meghashyama Prabhakara Bhat, Dattatraya Airodagi, Dhanyakumara Shivapoojar Basavarajappa, Muthuraj Rudrappa, Halaswamy Hiremath, Shashiraj Kareyelloppa Isolation, functional characterization, and eion Three hexose transporters (VvHT3, VvHT4, and VvHT5) were cloned from Vitis vinifera L. and functionally characterized in the hexose transport-impaired Saccharomyces cerevisiae mutant EBY.VW4000. Both VvHT4 and VvHT5 facilitated glucose uptake, with K(m)s of 137 muM and 89 muM, respectively. VvHT3 was not functional in the yeast system but a VvHT3:GFP (green fluorescent
Jun 11, 1999 · Isolation and Characterization of SRPP Gene. Two approaches, RT-PCR amplification and cDNA library screening, were employed to clone the gene. Sequence analysis showed that the genes isolated by the two different methods were identical except for minor differences in the untranslated regions (UTRs).